A Closer Look at 3-D Cell Cultures
It’s well known that
compared with 2-D
cell culture models,
3-D cell culture mod-
els have different pat-
terns of development,
to therapeutic targets and have different patterns of gene expression. Lawrence Berkeley National Labo-
ratory’s BioSig3D is the only computational platform that provides Web-based delivery of image-based
bioinformatics technology from 3-D cell culture models that are imaged in full 3-D using either confocal or
deconvolution microscopy. The computational platform is database-centric using PostgreSQL. All resources
and processes are registered, invoked and monitored by the database. Such resources also include Web pages
and an embedded workflow model that prompts the client for experimental design and annotation.
The system has six conceptual components that are tightly integrated through controlled vocabu-
laries (CVs), where the CVs have been borrowed and extended from existing ontologies. The Resource
Manager (RM) maintains an inventory of cell lines, reagents, antibodies and small molecule inhibitors.
The Experimental Design Manager (EDM) interacts with the RM, via querying the database, to design an
experiment utilizing a series of templates and CVs. The Data Load Manager supports three functionalities:
uploading data in a variety of input formats into the image server, linking data with associated experimental
factors and individual wells in a multiwall plate format and providing utilities for downloading images and
computed representations. Visualization modules support three distinct services: a thumbnail viewer of an
image stack, volume visualization and surface rendering of processed samples.
◗ Lawrence Berkeley National Laboratory, www.lbl.gov
Gold Standard for
Laparoscopic surgery was introduced with a goal
to reduce morbidities associated with open surgical techniques. Twenty years later, although it has
brought much better outcomes across a number
of indicators, it still has some significant patient
morbidities and mortality risks associated with it.
Port site hernia is one such example. No ubiquitous
global device-based standard of care developed has
been accepted to deal with this issue.
neoSurgical’s neoClose brings a simple, accurate technology/device-based solution that can be
deployed in less than 30 sec. neoClose facilitates
a new method type of port site closure referred
to as Vector X Closure, which is facilitated by the
delivery of two absorbable AutoAnchors, via the
AnchorGuide, through the abdominal wall.
The AutoAnchors are comprised of a PLGA
Anchor and PGA suture and are created by securing
the suture to the anchor via a proprietary process
that ensures attachment. After the AutoAnchors
have been deployed and the incision site is closed,
the AutoAnchors maintain tension at the port site
during the critical healing period of two weeks. At 14
days, the AutoAnchors maintain 50% of their initial
strength. The product is fully absorbed in approximately 12 months. The bioabsorbable polymers
degrade via a process called hydrolysis.
◗ neoSurgical, www.neosurgical.com
LFA Technology Sniffs Out Anthrax
Anthrax poses a significant threat to national security as demonstrated by terrorist attacks targeting the U.S. Postal Service and the Hart Senate Office Building. The organism that causes anthrax infection, Bacillus
anthracis (B. anthracis), is ubiquitous worldwide.
Anthrax outbreaks occur naturally in livestock. From
2005 through 2012, over 3,000 outbreaks were reported. In
resource-limited regions, suspicious samples are collected,
grown in a laboratory and analyzed by cell biology techniques.
Positive specimens are often stored, not destroyed. This
practice increases repositories of B. anthracis, escalating the
risk that it can be stolen for nefarious purposes. This practice
also leads trained laboratory personnel to produce pure B.
anthracis, a knowledge of which could be used for terrorism.
To mitigate these risks, Sandia National Laboratories developed a cred-it-card sized device based on the lateral flow assay (LFA) for detection of B.
anthracis in ultra-low resource environments: BaDx
(Bacillus anthracis diagnostics). BaDx is a low-cost, disposable device that requires no power, instrumentation or
equipment to operate, and no refrigeration to maintain
efficacy. It requires little training to operate, detecting
B. anthracis in the field with an accuracy that rivals laboratory analysis. Further, the device “self-destructs” by
sterilizing all contents upon assay completion. This greatly
improves safety for the operator, while preventing the dangerous bacteria from being recovered.
◗ Sandia National Laboratories, www.sandia.gov
Noninvasive Esophageal Cancer Imaging
There are two forms of esophageal cancer: squamous cell carcinoma and adenocarcinoma. Adenocarcinoma is increasing at a rate greater than all other cancers in the U.S. In fact, the American Cancer Society
estimated that in 2013, nearly 18,000 new cases of esophageal cancer cases will be diagnosed and more
than 15,000 people will die from the condition.
Currently, imaging and screening practices of esophageal tissue are time consuming and costly with
Farm Design have developed a solution, the NvisionVLE imaging system.
questionable effectiveness. For the patient, this process is also invasive. However, NinePoint Medical and
The NvisionVLE is a convergence of optical imaging, innovative engineering techniques, user-focused experience design and information visualization technology. At the heart of the system, Advanced Optical Coherence
Tomography (OCT) technology provides physicians and pathologists with comprehensive, volumetric digital
images of a patient’s esophagus in almost microscopic detail, at depths of up to 3 mm below the surface of the
esophageal tissue, and offers these images in real time. The NvisionVLE creates more complete cross-sectional
images, letting users evaluate 100% of the tissue in a 6-cm scan to better inform the patient evaluation. The scan
results are displayed on a 30-in high-resolution LCD touchscreen that allows the physician to zoom in on targeted
areas of the esophagus as the scan is done and capture and download specific images for later study.
◗ Nine Point Medical, www.ninepointmedical.com