Researchers often start an
experiment with assay-ready
cell plates of batches of cells
from a single suspension, which
often settles. Cell suspension
modules keep large batches of
cells continually in suspension
for high-throughput plating
experiments, saving time and
improving cell viability.
Media fill modules
Automated cell culture systems also require large volumes of fresh media
from a sterile source container to be delivered to the pipetting platform
when performing media exchanges. Media fill modules, such as the one
offered by Hamilton Robotics, can deliver warmed media to the pipetting workspace through a disposable fluid path connected to a source
container ( 4 C) located off-deck.
To maintain clean air over the workspace and protect sterile cell cultures,
manufacturers offer a variety of hoods. The best are HEPA-filtered hoods
that connect directly to the liquid handler. Ideally, a liquid handling platform
also integrates with biosafety cabinets for additional protection.
Integration with third-party devices, such as incubators, is important for
a fully automated, end-to-end, cell-based production system. Incubators control important parameters of cell growth by maintaining proper
growing temperatures, pH control and humidity. These devices also offer
decontamination routines and can accommodate many different sample
capacities and sample formats (tube or plate).
Automated cell counting saves time and is necessary for large-scale, cell-based
production systems. Cytometers easily integrate with high-quality liquid handling platforms and are critical in monitoring cell health and viability, and in
determining cell confluence, which indicates when to passage cells.
Other accessories include tilt modules that enable complete media and
cell removal during aspiration steps for passaging and harvesting, and heater shakers that assist in dissociating adherent cells from culture vessels.
Cell culture automation increasingly instrumental
With the increasing use of live cells for biological research, therapies and
drug discovery, automated cell culture systems are becoming even more
instrumental in solving the large bottlenecks associated with manual cell
These newer application-based systems can completely control and track
all cell culture processes, increase throughput and reduce labor costs, all
while further enhancing researchers’ ability to understand and treat disease.
—Kristina Klette, Don Janezic and Bobby Chavli
Heater shaker module for heating and
assisted adherent cell passaging.
LESS NOISE, LESS DRAFT
LabSox delivers 0RUH;$LUÁ;RZ with Less Noise and
Less Draft for Clean and Critical Environments,
while providing Flexible;SURGXFW;FRQÀ;JXUDWLRQV;IRU;
even the most challenging projects.