labeling step and increase throughout without eroding performance.
Size-exclusion chromatography (SEC) is used for protein sizing and
studying aggregates and bioconjugates, which are biomolecules that share
a covalent link. Even at the lowest concentrations, these “misfolded” proteins that accumulate together in recombinant protein and monoclonal
antibody biologics can be toxic and cause disease-producing effects. They
must be identified and remediated at every phase of the drug development lifecycle. Agilent Technologies Inc., Santa Clara, Calif., has introduced the 1260 Infinity Multi-Detector Bio-SEC solution, a SEC system
that features advanced light-scattering detection capabilities, fully bio-in-ert instrumentation, high-resolution columns and intuitive software.
These elements enhance SEC for protein aggregate analysis.
The 1260 Infinity Multi-Detector Bio-SEC solution provides high sensitivity and accuracy through advanced detection capabilities and sophisticated yet intuitive software capabilities. All capillaries and fittings throughout
the autosampler, column compartment and detectors are metal-free, so
the biomolecules in a user’s sample come in contact only with ceramics or
PEEK (polyether ether ketone). The dual-angle light-scattering detector
measures the scattering of monochromatic laser light by polymer molecules
at 15- and 90-degree angles. Combined with data from a concentration
detector, light-scattering detection gives absolute molecular weights without
the need for column calibration, accurate assessment of molecular size and
radius of gyration and direct determination of long-chain branching. Used
throughout the drug development lifecycle, the technology can simplify and
speed up workflow, reducing the time and expense associated with bringing
biopharmaceuticals to market.
GC for the masses
Commonly used in analytical chemistry for separating and analyzing
compounds that can be vaporized without decomposition, gas chromatography (GC) is typically applied to the purity testing of substances or
separating the different components of a mixture, especially within the life
science arena. GC can also help identify a compound; and in preparative
chromatography, GC can be used to prepare pure compounds from a mixture. In this method, the mobile phase is a carrier gas (helium or nitrogen)
and the stationary phase is a microscopic layer of liquid on an inert solid
support inside a column. Physical components of a GC system include
autosamplers, inlets, detectors and columns.
A new solution for the identification of unknowns in metabolomics
and small-molecule research, Bruker announced its GC-APC II source at
Efficient control of the heating of the transfer line preserves the GC
separation of Bruker’s 436-GC and 456-GC. The redesigned ionization
chamber provides high sensitivity and low background noise in combina-
tion with well-resolved GC peaks, delivering improved dynamic range and
up to tenfold gains in sensitivity. The interface can be combined with any
Bruker electrospray ionization (ESI) mass spectrometer and its unique
calibrant reservoir enables software-controlled calibrant delivery. The cali-
brant can be added for data-file recalibration in each GC run.
Expanding the horizons of fast and transportable GC applications with
innovations needed for accurate on-site analysis, INFICON, East Syracuse, N. Y., introduced its Micro GC Fusion at Pittcon 2014, the first temperature-programmable micro GC that offers fast temperature ramping
using capillary columns. Built on the company’s proven micro GC technology, coupled with rapid temperature ramping, the Micro GC Fusion
covers up to C12 gas phase analysis with excellent sensitivity and repeatability. The Micro GC system is equipped with a microelectromechanical
system (MEMS) thermal conductivity detector (TCD) that offers a 1 ppm
detection limit. The temperature-programmable GC column focuses late
eluting peaks, providing advanced control over peak resolution and significant sensitivity gains for heavier hydrocarbons.
The Micro GC system is based on a modular GC design, where each
module is comprised of an injector, a temperature-programmable capillary column and a detector. The instrument can be outfitted with one or
two modules to meet most application needs. Users can easily exchange
a GC module on-site in minutes to quickly adapt to new applications
or conduct instrument maintenance. The modules can also be easily
adaptable for OEM integration. The MEMS injector in the GC module
eliminates the complex valve-switching programming that is typical in a
benchtop GC, and the integrated sample conditioner in the instrument
allows the user to accurately analyze sample gas streams at input pressures of up to 1,000 psi. The system runs on browser-based software, is
equipped with a multi-touch front panel display and wireless network
protocol and has an interface that can adapt to any display dimension,
allowing users to operate the micro GC from a tablet, laptop or desktop
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together is progress. Working together is
success. CONSCI works with you to provide
custom state-of-the-art independent gas analysis
services such as ppb and ppt level volatile metal
impurities in specialty gases, trace impurities in
hard to manage samples such as metal hydrides,
complex mixtures and corrosive gases, as well as
fast and affordable routine impurity analyses.
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unique solutions to analytical challenges.