Life Sciences Chrome
Liquid and gas chromatography have become staples in medical, proteomics,
metabolomics and genomics applications.
Driven by rapid growth in forensics, biotechnology, disease diagnos- tics and environmental regulations, chromatography systems have become a laboratory staple. Used for the separation of complex mixtures, detection of illicit drugs and the production of pharmaceuticals, the biotechnology and pharmaceutical industries are the
prime users of chromatography techniques. Globally, as these industries
are expanding, the demand for these techniques and instrumentation is
increasing. Growth in the use of chromatography techniques in research
activities in the fields of medicine, proteomics, metabolomics and genomics also propels the growth of the market.
According to Transparency Market Research data, the global chromatography market is expected to grow at a CAGR of 4.1% from $6.6 billion in 2011
to $8.9 billion in 2017. The global chromatography accessories and consumables market, according to a Research and
Markets report, is an ever-changing tech-nology-driven market—which includes
columns, autosamplers, vials and detectors—and was valued at an estimated $2.6
billion in 2013, and is forecast to grow
at a CAGR of 5.7%, to reach $3.5 billion
Chromatography is categorized
into three types comprised of liquid,
gas and other chromatography techniques—which includes ion exchange
and flash. While in most economies the chromatography market is shifting towards advanced chromatographic technologies, like ion exchange,
liquid and gas chromatography will continue to sustain in the market.
These techniques are still main staples in most forensics, life science and
biotechnology laboratories. And this trend is highlighted through many
products offered by Agilent Technologies, Phenomenex, Thermo Fisher
Scientific, Shimadzu Scientific Instruments, Waters Corp. and more.
LC enhances life science separations
Liquid chromatography (LC) is a separation technique in which the mobile
phase is a liquid. The technique can be conducted either in a column or a
plane and generally uses small packing particles and relatively high pressure.
Chromatographic separations of proteins are based on their difference either
in size (size-exclusion chromatography), electric charges (ion-exchange
chromatography) or hydrophobicity (reversed-phase chromatography).
JM Science offers a complete line of Shiseido HPLC CAPCELL PAK
Columns with the Proteonavi HPLC column to separate proteins and
peptides in reversed-phase mode. In reversed-phase mode, which uses a
hydrophobic stationary phase, it’s known that proteins and peptides with
higher-order structure are denatured in the course of retainment on the
stationary phase after introduction to the column. Once the retention
with denaturation occurs, molecules won’t migrate until the organic con-
tent of the mobile phase is raised to an appropriate level under a gradient
program. Proteonavi utilizes high-purity silica with few metal impurities,
and shows minimal irreversible adsorption for proteins and peptides. Its
pore size is as wide as 30 nm, enabling large proteins enough interactions
with the stationary phase.
The primary source of protein retention on reversed phase is hydrophobic interaction between hydrophobic parts of amino acid residues and
alkyl chains in the stationary phase. When secondary interactions, such
as coulombic interactions by acidic silanols (silica compounds) or metal
impurities in the stationary phase, exist to a large extent, peak shapes of
proteins will deteriorate due to their slow kinetics. Proteonavi allows better peak profiles and higher resolution among standard proteins in comparison with conventional wide-pore columns. This system is suitable for
large-scale preparative separations.
For laboratories looking to produce greater amounts of information
faster with chromatographic separations, Waters Corp. (Milford,
Mass.) recently released the Waters CORTECS columns, a
new family of 1.6-µm solid-core UltraPerformance
LC (UPLC) columns. The column particles
feature a solid, impermeable silica
core encased in a porous
silica outer layer where
the interactions between
the stationary phase and the
analytes occur. Available in C18,
C18+ or HILIC (hydrophilic interaction
chromatography) chemistries, the column offerings include 30 configu-
rations. The CORTECS C18 column is a general-purpose, high-efficiency,
reversed-phase column offering balanced retention of acids, bases and
neutrals at low and mid-range pH. The CORTECS C18+ column is a
general-purpose reversed-phase column with a positively charged surface
that delivers excellent peak shape for basic compounds at low pH. And the
CORTECS HILIC column is designed for the retention of extremely polar
analytes, while offering orthogonal selectivity versus C18 columns.
The growing number of scientists investigating glycans as disease
markers and antibody-based biopharmaceuticals now have an effective
new tool for the challenging separations of glycans and glycan isomers. Until now, glycan isomers couldn’t be separated by conventional
high-performance liquid chromatography (HPLC), but Thermo Fisher
Scientific, Waltham, Mass., has introduced the GlycanPac AXR-1 column,
which helps users achieve greater resolution of glycans.
These columns are designed for high resolution of labeled and unlabeled
glycans, and are compatible with fluorescence and mass spectrometry detection methods. Mass spectrometry (MS) has emerged as a powerful tool for
determining the structures of glycans, and the GlycanPac AXR-1 column
is designed to enable researchers to harness the power of high-resolution
accurate-mass MS at high-throughput rates. The column is also designed to
separate both labeled and native glycans based on the availability of samples.
Native glycan separation allows researchers to eliminate the fluorescent
has introduced the
GlycanPac AXR-1 column,
which helps users achieve greater
resolution of glycans using HPLC.